Overexpression of GILZ in macrophages limits systemic inflammation while increasing bacterial clearance in sepsis in mice - Université Paris-Est-Créteil-Val-de-Marne Access content directly
Journal Articles European Journal of Immunology Year : 2020

Overexpression of GILZ in macrophages limits systemic inflammation while increasing bacterial clearance in sepsis in mice

Mehdi Ellouze
  • Function : Author
Lola Vigouroux
  • Function : Author
Colas Tcherakian
  • Function : Author
Paul‐louis Woerther
Aurélie Guguin
  • Function : Author
Olivier Robert
  • Function : Author
Mathieu Surenaud
Thi Tran
  • Function : Author
Joseph Calmette
  • Function : Author
Thomas Barbin
  • Function : Author
Gabriel Perlemuter
Anne‐marie Cassard
  • Function : Author
Pierre Launay
Djillali Annane
Yves Levy
  • Function : Author
Véronique Godot

Abstract

Abstract Studies support the beneficial effects of glucocorticoids (GCs) during septic shock, steering research toward the potential role of GC‐induced proteins in controlling excessive inflammatory responses. GILZ is a glucocorticoid‐induced protein involved in the anti‐inflammatory effects of GCs. We investigated whether the overexpression of GILZ specifically limited to monocytes and macrophages (M/M) alone could control inflammation, thus improving the outcome of septic shock in animal models. We also monitored the expression of GILZ in M/M from septic mice and septic‐shock patients. M/M from patients and septic mice displayed significantly lower expression of GILZ than those isolated from controls. Furthermore, transgenic mice (Tg‐mice) experiencing sepsis, with increased expression of GILZ restricted to M/M, showed lower frequencies of inflammatory monocytes than their littermates and lower plasma levels of inflammatory cytokines. Tg‐mice also had lower blood bacterial counts. We further established that the upregulation of GILZ in M/M enhanced their phagocytic capacity in in vivo assays. The increase of GILZ in M/M was also sufficient to improve the survival rates of septic mice. These results provide evidence for a central role of both GILZ and M/M in the pathophysiology of septic shock and a possible clue for the modulation of inflammation in this disease.

Dates and versions

hal-04298575 , version 1 (21-11-2023)

Identifiers

Cite

Mehdi Ellouze, Lola Vigouroux, Colas Tcherakian, Paul‐louis Woerther, Aurélie Guguin, et al.. Overexpression of GILZ in macrophages limits systemic inflammation while increasing bacterial clearance in sepsis in mice. European Journal of Immunology, 2020, 50 (4), pp.589-602. ⟨10.1002/eji.201948278⟩. ⟨hal-04298575⟩
5 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More