Biometric systems are considered an efficient component for identification in the developing modern technologies. The aim of biometric systems is to verify or determine the identity of a user through his/her biological and behavioral characteristics. The threat of spoof attacks is always an important issue in biometric verification and authentication, which requires an updated and stronger protection system. In this article, we propose an anti-spoofing system based on auditory perception responses. To the best of our knowledge, this is the first time that an auditory perception based anti-spoofing system has been presented for age verification. The proposed auditory perception based anti-spoofing system was evaluated with 770 trials conducted by many subjects of each gender and age range (12–65 years of age). The results achieved are encouraging, as the auditory perception based system showed the lowest Equal Error Rate (EER) value of 5.5%.