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Journal Articles Advances in Microbial Physiology Year : 2011

Galleria mellonella as aModel for Studying Enterococcus faecium Host Persistence

François Lebreton
  • Function : Author
Florian Le Bras
  • Function : Author
Fany Reffuveille
  • Function : Author
Rabia Ladjouzi
Jean-Christophe Giard
  • Function : Author
Roland Leclercq
  • Function : Author
Vincent Cattoir
  • Function : Author


Enterococcus faecium is an opportunistic pathogen responsible for numerous outbreaks worldwide. The basis for the colonization capacities, host persistence and environmental stress response of the hospital-adapted clones emerging from E. faecium are poorly understood. In this study, we propose the use of Galleriamellonella as a simple nonmammalian model to assess E. faecium host persistence. Various strains (n = 10), including hospital-adapted, commensal or animal isolates and a SodA-deficient strain were used to assess the relevance of this model. Compared to Enterococcus faecalis, E. faecium strains do not appear very lethal in a Galleria killing assay. The ability of E. faecium strains to overcome host-immune responses and multiply within the host system was evaluated by monitoring bacterial loads following Galleria infection. Among the E. faecium strains, two hospital-adapted isolates displayed increased colonization ability. In contrast, inactivation of sodA, encoding a putative manganese-dependent superoxide dismutase, significantly reduced survival of E. faecium to Galleria defenses. Galleria appears to be a suitable and convenient surrogate model to study E. faecium survival to host defenses and the role of suspected virulence factors in the colonization process.
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hal-04322073 , version 1 (04-12-2023)



François Lebreton, Florian Le Bras, Fany Reffuveille, Rabia Ladjouzi, Jean-Christophe Giard, et al.. Galleria mellonella as aModel for Studying Enterococcus faecium Host Persistence. Advances in Microbial Physiology, 2011, 21 (3-4), pp.191-196. ⟨10.1159/000332737⟩. ⟨hal-04322073⟩
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