Large amplitude motion of methyl groups in isolated molecules is a fundamental phenomenon in molecular physics. The methyl torsional barrier is sensitive to the steric and electronic environment in the surrounding of the methyl group, making the methyl group a detector of the molecular structure. To probe this effect, the microwave spectrum of 2,6-dimethylfluorobenzene, one of the six isomers of dimethylfluorobenzene, was measured using two pulsed molecular jet Fourier transform microwave spectrometers operating in the frequency range from 2 to 40 GHz. Due to internal rotations of two equivalent methyl groups with relatively low torsional barriers, all rotational transitions split into quartets with separations of up to several hundreds of MHz. The splittings were analyzed and modeled to deduce a torsional barrier of 236.7922(21) cm−1. The results are compared to those obtained from quantum chemical calculations and with other fluorine substituted toluene derivatives of the current literature where the methyl group is adjacent to the fluorine atom.