Glycerol dehydratase activity: the limiting step for 1,3-propanediol production by Clostridium butyricum DSM 5431 - Université Paris-Est-Créteil-Val-de-Marne Access content directly
Journal Articles Letters in Applied Microbiology Year : 1996

Glycerol dehydratase activity: the limiting step for 1,3-propanediol production by Clostridium butyricum DSM 5431

E. Guedon
  • Function : Author
E. Spiesser
  • Function : Author
H. Petitdemange
  • Function : Author

Abstract

S. ABBAD‐ANDALOUSSI, E. GUEDON, E. SPIESSER AND H. PETITDEMANGE. 1996. Glycerol catabolism by Clostridium butyricum DSM 5431 into acetate, butyrate and 1,3‐propanediol (1,3‐PD) was studied in chemostat culture. The fact that the intracellular concentrations of NADH (18–22 μUmol g‐1dry cell mass) were extremely high suggested that the dehydratase activity was the rate limiting step in 1,3‐PD formation. This limitation was proved by the addition of propionaldehyde, another substrate of propanediol dehydrogenase, into the culture medium. This resulted in an increase in (i) glycerol utilization, (ii) biomass formation and (iii) product biosynthesis.
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hal-04473324 , version 1 (22-02-2024)

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S. Abbad-Andaloussi, E. Guedon, E. Spiesser, H. Petitdemange. Glycerol dehydratase activity: the limiting step for 1,3-propanediol production by Clostridium butyricum DSM 5431. Letters in Applied Microbiology, 1996, 22 (4), pp.311-314. ⟨10.1111/j.1472-765X.1996.tb01168.x⟩. ⟨hal-04473324⟩
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